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BNCC

Shigella dysenteriae (Shiga) Castellani and Chalmers

SKU 291532

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismShigella dysenteriae (Shiga) Castellani and Chalmers
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 2-4 mm, circular with irregular margins, opaque, pale yellow on top, centrally convex, smooth surface, glossy appearance, moist texture, G- (red), bacillus, purity: pure
Culture Method① Prepare 1 tube containing 5–10 mL liquid medium or sterile water and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5mL liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once growth appears.
Culture Conditions37°C, 18-24h, aerobic
Provided FormatsAgar Slant; Bacterial suspension; Agar plate
Culture Medium No.369667
Culture Medium NameNutrient Broth (NB)
Culture Medium CompositionPeptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.