BNCC
Rubella virus
SKU 390494
Bioware
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Rubella virus |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Frozen vial |
| Culture Method | ① Inoculation procedure: 24-48 hours prior to inoculation, seed host cells in T25 flasks. Prepare for viral inoculation when host cell density reaches 80-90%; Remove the cell culture medium and inoculate 100-500μL of virus solution. Allow adsorption for 1-2 hours under culture conditions, gently shaking the T25 flask every 15-30 minutes to ensure even virus distribution. Add 6mL of virus growth medium to complete adsorption, then transfer to the incubator for continued culture. Regularly observe for cytopathic effects; ② Virus Harvesting: Initiate virus collection once host cells exhibit complete cytopathic changes. During collection, freeze-thaw the cells 1–2 times, then filter through a 0.22μm pore size filter. The collected filtrate constitutes the virus solution. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361988 |
| Culture Medium Name | EMEM Complete Medium (with 2% FBS) |
| Culture Medium Composition | 98% EMEM+2% FBS |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.