BNCC
Rotavirus A
SKU 362558
Bioware
Concentration
Product Code
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Rotavirus A |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Frozen vial |
| Culture Method | ①Inoculation procedure: 24-48 hours before inoculation, transfer the frozen host cells into T25 flask, remove the cell culture medium when the host cell density reaches 80-90%, and inoculate 100-500 μL of virus solution. Adsorb under growth conditions for 1-2 hours, gently shake the T25 flask every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cytopathy; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | Frozen vial |
| Culture Medium No. | 363231 |
| Culture Medium Name | VR-2018 Specific Medium |
| Culture Medium Composition | EMEM+2μg/mL Trypsin |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.