BNCC
Rosetta-gami(DE3)pLysS
SKU 357925
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Rosetta-gami(DE3)pLysS |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 1-2mm, circular, with neat edges, opaque, light yellow on the front, smooth and bright on the surface, moist texture, no pigment, G - (red), Bacillus, purity: pure |
| Culture Method | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μL, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Culture Conditions | 37 °C; 24 hours; aerobic |
| Provided Formats | Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 391487 |
| Culture Medium Name | LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, 50μg/mL Streptomycin, and 10μg/mL Tetracycline (Ready-to-use) |
| Culture Medium Composition | Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K2HPO4:2.0g, Tween 80:1.0ml, CaCl2.2H2O:0.01g, MgSO4.7H2O:0.02g, K2HPO4:0.04g, KH2PO4:0.04g, NaHCO3:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C3H7NO2S.HCl:0.5g, Rumen Fluid(30ml/L), pH:7.2±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.