BNCC
Quantitative strain of Bacillus subtilis
SKU 353859
Bioware
A standardized microbiological quality-control material designed to provide a defined analytical challenge.
Suitable for method verification, recovery studies, detection or enumeration performance assessment, and routine quality control.
Technical Specifications
| Organism | Quantitative strains of bacillus subtilis subsp. spizizenii Nakamura et al. |
|---|---|
| Material Type | Microbiological Quality Control |
| Preservation Form | freeze-dried bacterial tablets |
| Morphological Characteristics | The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, bright on the surface, moist in texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus, purity: Pure |
| Culture Method | ① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | penicillin bottle |
| Culture Medium No. | 352252 |
| Culture Medium Name | Brain Heart Infusion Agar |
| Culture Medium Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25°C) |
| Pack Configuration | Single Vial |
|---|
| Application | Microbiological method verification, enumeration, and laboratory quality control. |
|---|---|
| Storage Conditions | -20 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.