BNCC
pX459 V2.0
SKU 390539
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | pX459 V2.0 |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Culture Method | ①Dissolution: Add 20μL sterile water to the plasmid Freeze dried pellet and dissolve; let stand at Room temperature for 1 min; ②Mixing: Take 200μl competent cells and mix with 5-10μl plasmid DNA; place on ice for 30 min; ③Heat shock transformation: Incubate at 42°C for 90 sec; ④ Cooling: Place on ice for 2 min; ⑤ Growth: Add 800 μL liquid medium to each tube and incubate at 37°C on a shaker (150 rpm) for 1 h; ⑥ Screening culture: Agar plate an appropriate volume (100 μl) of thawed cells onto LB agar plates with the corresponding antibiotic. Incubate plates upright for 30 min (ensure agar surface is dry), then invert and incubate for 12-16 hours until colonies appear; ⑦ Extraction: Transfer a single colony to the corresponding antibiotic-containing LB liquid medium. Incubate with shaking for 12-16 hours. Extract the plasmid as required for the experiment. |
| Culture Conditions | LB + Ampicillin, 37°C (Cloning strain: Stbl3, Origin: U6, Repository: pUC) |
| Provided Formats | Freeze dried pellet |
| Culture Medium No. | 361029 |
| Culture Medium Name | LB Medium with 50μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.