pTRE3G — BNCC 354922

BNCC

pTRE3G

SKU 354922

Bioware

A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.

Technical Specifications

OrganismpTRE3G
Material TypeRecombinant Microbial Strain
Preservation FormFreeze dried pellet
Culture Method1. Dissolution: Upon receiving the plasmid Freeze dried pellet, add 20μl sterile water to the bottom of the tube to dissolve the plasmid. Allow to stand at Room temperature for 1 minute. 2. Mixing (Plasmid Adsorption): Mix 200μl competent cells + 5~10μl plasmid DNA thoroughly. Incubate on ice for 30 minutes. 3. Heat shock transformation: Incubate at 42°C for 90 seconds; 4. Shrink membrane wells: Ice-bathe for 2 minutes; 5. Repair culture: Add 800 µl LB liquid medium to each tube; incubate at 37°C for 1 hour at 150 rpm; 6. Screening culture: Spread an appropriate volume (100 µl) of the revived cells onto LB plates with the corresponding antibiotic. Incubate plates upright for 30 min (ensure agar surface is dry), then invert and incubate for 12–16 hours until colonies appear. 7. Extraction: Transfer a single colony to the corresponding antibiotic-supplemented LB liquid medium. Incubate with shaking for 12–16 hours. Extract the plasmid as required for the experiment.
Culture ConditionsLB + Ampicillin, 37°C (Cloning strain DH5α, 3431 bp, Promoter: TRE3G, Repository: pUC)
Provided FormatsFreeze dried pellet
Culture Medium No.361029
Culture Medium NameLB Medium with 50μg/mL Ampicillin
Culture Medium CompositionYeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C)
ApplicationMolecular biology research, recombinant DNA studies, method development, and laboratory research.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.