BNCC
Pseudomonas phage phi6 (Cystovirus phi6)
SKU 354296
Bioware
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Pseudomonas phage phi6 (Cystovirus phi6) |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Frozen vial |
| Culture Method | phage culture: ① Host bacterial preparation: pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL tryptone soy broth culture medium, shake at 30 °C, 160-180 rpm until OD600 is 0.4-0.6; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 30 °C water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 30 °C and shake at 160-180rpm for 48 hours; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and filter it with a 0.22 μ m sterile filter, and collect the filtrate. The filtrate can be stored at 4 °C |
| Culture Conditions | 30 °C; 24-48 hours; aerobic |
| Provided Formats | Bacterial solution |
| Culture Medium No. | 363070 |
| Culture Medium Name | Tryptic Soy Agar (TSA) |
| Culture Medium Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25°C) |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.