pLV[Exp]-EGFP-CMV>{NL-63-N} — BNCC 377855

BNCC

pLV[Exp]-EGFP-CMV>{NL-63-N}

SKU 377855

Bioware

A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.

Technical Specifications

OrganismpLV[Exp]-EGFP-CMV>{NL-63-N}
Material TypeRecombinant Microbial Strain
Preservation FormFrozen vial
Culture Method① Prepare one sterile water vial and two 90mm resistant plates; ② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then quickly add sterile water to rupture; break ampoule with forceps;③ Pipette 0.5 mL sterile water into the lyophilized tube. After complete dissolution, spread the solution onto antibiotic plates at 200 μL/Agar plate; ④ Incubate antibiotic plates under specified conditions for 18–24 hours before observation (do not seal or wrap plates);⑤ Pick a single colony and inoculate it into 50 mL of LB antibiotic-supplemented liquid medium. Incubate overnight at 37°C on a shaking incubator. ⑥ Depending on experimental requirements, aspirate an appropriate amount of bacterial suspension for plasmid extraction.
Culture ConditionsLB + antibiotics, 37°C
Provided FormatsFrozen vial
ApplicationMolecular biology research, recombinant DNA studies, method development, and laboratory research.
Storage Conditions4°C, 30 days; -20°C, 12 months.
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.