BNCC
pETBlue-2
SKU 358817
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | pETBlue-2 |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Culture Method | ① Dissolve: Add 20 μL of sterile water to the plasmid powder and let it stand at Room temperature for 1 minute; ② Mixing: Mix 200 μ l of competent cells with 5-10 μ l of plasmid DNA and place on ice for 30 minutes; ③ Heat shock introduction: let stand at 42 °C for 90 seconds; ④ Shrinkage film pores: ice bath for 2 minutes; ⑤ Repair culture: Add 800 μ l of liquid culture medium to each tube and culture on a shaker at 37 °C for 1 hour (150 r/min); ⑥ Screening and cultivation: Apply an appropriate volume (100 μ l) of revived cells onto corresponding resistant LB plates, place them upright in a petri dish for 30 minutes (the agar surface must be dry), invert and culture for 12-16 hours, and colonies will appear; ⑦ Extraction: Select monoclonal colonies into the corresponding resistant LB liquid medium, shake and culture for 12-16 hours, and extract plasmids according to experimental needs. |
| Culture Conditions | 37°C； 18-24 hours; aerobic |
| Provided Formats | Freeze dried pellet |
| Culture Medium No. | 362905 |
| Culture Medium Name | LB Medium with 100μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.