BNCC
pET28a-EGFP
SKU 342269
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | pET28a-EGFP |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Culture Method | 1. Dissolution: After receiving the plasmid powder, centrifuge at 12000r/min for 30 seconds. Add 20 μ l of sterile water to the bottom of the tube, dissolve the plasmid, and let it stand at Room temperature for 1 minute; 2. Mixing (adsorption plasmid): Mix 200 μ l of competent cells and 5-10 µ l of plasmid DNA, and place on ice for 30 minutes; 3. Heat shock introduction: let stand at 42 °C for 90 seconds; 4. Shrinkage film pores: ice bath for 2 minutes; 5. Repair culture: Add 800 µ l LB liquid medium to each tube and incubate at 37 °C for 1 hour at 150 r/min; 6. Screening and cultivation: Apply an appropriate volume (100 µ l) of revived cells onto corresponding resistant LB plates, place them upright in the Agar plate for 30 minutes (the agar surface must be dry), invert and culture for 12-16 hours, and colonies will appear. 7. Extraction: Select monoclonal colonies into the corresponding resistant LB liquid medium, shake and culture for 12-16 hours, and extract plasmids according to experimental needs. |
| Culture Conditions | 37 °C, 18-24h, aerobic |
| Provided Formats | Freeze dried pellet |
| Culture Medium No. | 361023 |
| Culture Medium Name | LB Medium with 50μg/mL Kanamycin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.