BNCC
PBMC – Peripheral blood mononuclear cells
SKU 392533
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | PBMC |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Single cells |
| Culture Method | Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 °C refrigerator and place it in PE gloves. Quickly immerse it in a 37 °C water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 6-7mL of complete medium and place the flask vertically in an incubator. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | cryovial |
| Culture Medium No. | 379583 |
| Culture Medium Name | P3HR-1 Cell-Specific Medium |
| Culture Medium Composition | 80% RPMI-1640 + 20% Heat-Inactivated FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.