BNCC
P815 – Mouse mast cell tumor cells
SKU 100552
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | P815 |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Lymphoblast-like，Spindle-shaped (short)，Round，irregular edge |
| Culture Method | Recovery steps: ① Remove the cryovial from liquid nitrogen or -80 °C freezer and place it in PE gloves. Quickly immerse it in a 37 °C water bath and shake the cryovial to accelerate dissolution. It is recommended to dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 6-8mL of complete culture medium and culture it in an incubator.Cell passage: ① Gently scrape off the adherent cells with a cell scraper, transfer them along with the culture medium to a centrifuge tube, centrifuge at 1000 RPM for 5-8 minutes, and discard the supernatant; ② Add 1-2 mL of complete culture medium to the cell pellet collected from suspended cells and adherent cells, and mix well; ③ Add culture medium at a rate of 5-6mL/bottle, and divide the cell suspension into culture bottles containing 5-6mL of culture medium at a ratio of 1:2; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | cryovial; T25 culture flask |
| Culture Medium No. | 338068 |
| Culture Medium Name | DMEM-H Complete Medium (with 10% FBS) |
| Culture Medium Composition | 90%DMEM-H+10%FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.