BNCC
NCI-H1944 – Human non-small cell lung cancer cells
SKU 359250
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | NCI-H1944 |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Epithelial-like，irregular edge，Monolayer adherent growth |
| Culture Method | Resuscitation steps: ① Remove the Frozen vial from liquid nitrogen or -80 °C and place it in a PE glove. Quickly immerse it in a 37 °C water bath, shake the Frozen vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9ml of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 5-6mL of complete culture medium and culture it in an incubator. Cell passage: ① Remove the old culture medium, wash twice with PBS, and add 1-2mL Trypsin (0.25% Trypsin + 0.02% EDTA); ② Observe the digestion process under the microscope. When the cell edge shrinks and the adhesion becomes loose (you can use a straw to suck up some trypsin and gently blow it to a certain part of the cell layer. The cell layer can be seen to have fallen off with the naked eye, indicating that digestion is complete. Otherwise, continue digestion). Directly suck out the trypsin, add 5-6 milliliters of complete culture medium, gently blow the cell layer, blow it off, and disperse. ③ Divide the cell suspension into new T25 bottles at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator. ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | cryovial; T25 culture flask |
| Culture Medium No. | 338360 |
| Culture Medium Name | RPMI-1640 Complete Medium (with 10% FBS) |
| Culture Medium Composition | 90%RPMI-1640+10%FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.