BNCC
Mycobacterium ulcerans MacCallum et al.
SKU 368043
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium ulcerans MacCallum et al. |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial tube |
| Morphological Characteristics | The liquid culture medium is turbid, the agar layer is obvious, the acid fast staining is positive, the bacteria are pure, and the purity is pure |
| Culture Method | ① Prepare 1 bottle of liquid culture medium, about 200mL; ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid culture medium; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Culture Conditions | 30 °C; From February to June; aerobic; (Transfer and culture the bacterial solution after rejuvenation for 2 months) |
| Provided Formats | Bacterial suspension |
| Culture Medium No. | 360851 |
| Culture Medium Name | Middlebrook 7H10 Agar |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.