BNCC
Mycobacterium tuberculosis (Zopf) Lehmann and Neumann H37Ra
SKU 263834
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium tuberculosis (Zopf) Lehmann and Neumann H37Ra |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony has a diameter of 0.5-1mm, irregular shape, irregular edges, yellow on the front, light color, raised in the middle, rough surface, and dry texture, G＋ |
| Culture Method | ① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μL of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. During the cultivation process, the agar plate needs to be kept moist. |
| Culture Conditions | 37 ° C; 4-5 weeks; aerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 360851 |
| Culture Medium Name | Middlebrook 7H10 Agar |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.