BNCC
Mycobacterium tuberculosis variant bovis BCG TMC 1022 [BCG Russian]
SKU 394000
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium tuberculosis variant bovis BCG TMC 1022 [BCG Russian] |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 1-2mm, the edges are irregular, opaque, the front is white, the middle is raised, the surface is rough, the surface is wrinkled, the texture is hard, the texture is dry, acid resistant staining is positive, Bacillus, purity: pure |
| Culture Method | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation, and the strain can be used once it grows (the agar plate needs to be kept moist during the cultivation process). |
| Culture Conditions | 37 ° C; 3-4 weeks; aerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 360851 |
| Culture Medium Name | Middlebrook 7H10 Agar |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.