BNCC

Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur]

Name: Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur]
Brand: BNCC
SKU: 364770
Availability: InStock

SKU 364770

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

Organism	Mycobacterium tuberculosis variant bovis BCG TMC 1011 [BCG Pasteur]
Material Type	Microbial Strain
Preservation Form	Freeze dried pellet
Morphological Characteristics	Pale yellow granular, hard texture, difficult to pick, rod-shaped
Culture Method	① Prepare the above two tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation, and the strain can be used once it grows (the agar plate needs to be kept moist during the cultivation process).
Culture Conditions	37 ° C; 3-4 weeks; aerobic
Provided Formats	Freeze dried pellet; Bacterial suspension; Agar plate
Culture Medium No.	360851
Culture Medium Name	Middlebrook 7H10 Agar
Culture Medium Composition	(NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C)

Application	Microbiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions	2-8 °C
Country of Origin	China

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.