BNCC
Mycobacterium simiae
SKU 365102
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium simiae |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colonies measure 0.5-1 mm in diameter and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth in texture, making them easy to lift. The organisms are acid‑fast stain positive. Purity: pure. |
| Culture Method | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μL per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. During the cultivation process, the agar plate should be kept moist. After the agar plate is dry, it can be opened in a safe cabinet and an appropriate amount of liquid medium containing additives should be added. The colony diameter is 0.5-1mm, opaque, yellow on the front, light in color, protruding in the middle, smooth on the surface, easy to pick up, and positive for acid resistant staining, |
| Culture Conditions | 37 °C; 5-7 days; aerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 360851 |
| Culture Medium Name | Middlebrook 7H10 Agar |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.