Skip to content
Mycobacterium haemophilum Sompolinsky et al. — BNCC 372357

BNCC

Mycobacterium haemophilum Sompolinsky et al.

SKU 372357

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismMycobacterium haemophilum Sompolinsky et al.
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsGranular, gray white, rough surface, wrinkled surface, hard texture, rod-shaped
Culture Method① Prepare the above 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Cultivate the agar plate under the above cultivation conditions, and the strain can be used once it grows (note: the agar plate needs to be kept moist during the cultivation process, and 7H10 broth can be added).
Culture Conditions30 ° C; 4-5 weeks; aerobic
Provided FormatsFreeze dried pellet; Bacterial suspension; Agar plate
Culture Medium No.360851
Culture Medium NameMiddlebrook 7H10 Agar
Culture Medium Composition(NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.