BNCC
Mycobacterium bovis Karlson and Lessel
SKU 339446
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium bovis Karlson and Lessel |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure |
| Culture Method | ① Prepare the above two tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation, and the strain can be used once it grows (during the cultivation process, the agar plate needs to be kept moist, and 7H9 broth can be added). |
| Culture Conditions | 37 ° C; 3-4 weeks; aerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 375932 |
| Culture Medium Name | Middlebrook 7H9 Broth |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.0g, Na2HPO4:2.5g, Na3C6H5O7:0.1g, MgSO4:0.05g, CaCl2:0.0005g, ZnSO4:0.001g, CuSO4:0.001g, L-Glutamic acid(C5H9NO4):0.5g, C6H8FeNO7:0.04g, Vitamin B6(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.0005g, ADC enrichment:10%(added at 50°C), pH:6.6±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.