BNCC
Mycobacterium avium subsp. paratuberculosis
SKU 364294
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Mycobacterium avium subsp. paratuberculosis |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Morphological Characteristics | The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure |
| Culture Method | ① Dissolve the frozen tube in a 37 °C water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μL per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. During the cultivation process, the agar plate needs to be kept moist, and 7H10 broth can be added. |
| Culture Conditions | 37 °C; 7-10 days; aerobic |
| Provided Formats | Bacterial suspension; Agar plate |
| Culture Medium No. | 360851 |
| Culture Medium Name | Middlebrook 7H10 Agar |
| Culture Medium Composition | (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50°C), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.