BNCC
M13KO7 Helper Phage
SKU 383941
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | M13KO7 Helper Phage |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | host culture: 37 °C, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 20.0g agar (excluding liquid medium), 1.0L distilled water, pH 7.0. Sterilize at 121 °C for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake at 37 °C, 160-180 rpm until OD600 is 0.1-0.4; ② Transfer: Take an appropriate amount of bacterial solution and transfer it into a triangular bottle of host bacterial solution; ③ Cultivation: Place the triangular flask at 37 °C and incubate overnight on a shaker at 160-180rpm; ④ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes, filter the lysate using a 0.22 μ m sterile filter, and store the filtrate at 4 °C |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | bacterial solution |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.