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BNCC

Listeria monocytogenes

SKU 185988

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismListeria monocytogenes
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis
Culture Method① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Culture Conditions37 °C; 18-24h; aerobic
Provided FormatsFreeze dried pellet; Agar Slant; Bacterial suspension; Agar plate
Culture Medium No.352252
Culture Medium NameBrain Heart Infusion Agar
Culture Medium CompositionBeef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.