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Legionella pneumophila subsp. pneumophila — BNCC 364771

BNCC

Legionella pneumophila subsp. pneumophila

SKU 364771

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLegionella pneumophila subsp. pneumophila
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 1-2 mm; edges: regular and opaque; upper surface: white, convex; texture: moist; pigment-negative; Gram-negative (red); bacillus; purity: pure
Culture Method① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μL per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Culture Conditions37 °C; 48-72h; 5% CO2
Provided FormatsFreeze dried pellet; Agar plate
Culture Medium No.360928
Culture Medium NameBuffered Charcoal Yeast Extract Agar
Culture Medium CompositionYeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C5H5KO5):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe4(P2O7)3):0.25g
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.