BNCC

Legionella pneumophila subsp.pneumophila Brenner et al.

SKU 312197

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLegionella pneumophila subsp.pneumophila Brenner et al.
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter 0.5-2 mm, circular with irregular margins, opaque, grayish-white on the obverse, convex in the center, smooth and glistening surface, moist and viscous texture, Gram-negative (red), rod-shaped, purity: pure.
Culture Method① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Culture Conditions37 °C; 24-48h；5%CO2；
Provided FormatsFreeze dried pellet; Agar plate
Culture Medium No.360928
Culture Medium NameBuffered Charcoal Yeast Extract Agar
Culture Medium CompositionYeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C5H5KO5):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe4(P2O7)3):0.25g
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.