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BNCC

Lactobacillus delbrueckii subsp. lactis

SKU 289804

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLactobacillus delbrueckii subsp. lactis
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsFor Gram positive bacteria, the MRS liquid culture medium is turbid, with a large amount of sediment at the bottom of the test tube. The growth of the Agar plate strain is weak, and it is recommended to use liquid culture
Culture Method① Prepare 1 liquid culture medium with a diameter of 18mm, approximately 10mL; ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Culture Conditions37 °C; 24-48 hours; 5% CO2+95% air
Provided FormatsFreeze dried pellet; Bacterial suspension
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.