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BNCC

Lactobacillus delbrueckii subsp. bulgaricus

SKU 186626

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLactobacillus delbrueckii subsp. bulgaricus
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe bacterial culture in MRS broth appears turbid with a significant amount of white sediment at the bottom of the tube. The growth of the strain on the Agar plate is weak; therefore, using liquid culture is recommended.
Culture Method① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μL, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Culture Conditions37 °C; 2-3 days; Micro aerobic gas environment (3% -5% O2, 10% CO2)
Provided FormatsFreeze dried pellet; Bacterial suspension
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.