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BNCC

Lactobacillus crispatus

SKU 395782

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLactobacillus crispatus
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColonies are 1–2 mm in diameter, circular, with smooth, well-defined edges; they are opaque, milky white on the upper surface, convex in the center, with a smooth, lustrous surface and a moist texture. They do not produce pigments, are G+ (blue-violet), rod-shaped, and have a purity of: pure
Culture Method① Prepare 1–2 of the above agar plates; ② Open them in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to crack the surface, and subsequently crush the contents with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the strain is ready for use once colonies appear.
Culture Conditions37°C; 48–72 h; anaerobic
Provided FormatsFreeze dried pellet; bacterial suspension; plate culture
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2–8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.