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BNCC

Lactobacillus brevis

SKU 189801

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLactobacillus brevis
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony Diameter: 1-2 mm Shape: Circular Margin: Entire Transparency: Opaque Color (Front): Grayish white Elevation: Raised center Surface: Smooth and shiny Texture: Moist and viscous Gram Stain: Gram-positive (blue-purple), rod-shaped (bacilli) Purity: Pure
Culture Method① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Culture Conditions37 °C; 48-72 hours; microaerophilic (3% -5% O2, 10% CO2)
Provided FormatsFreeze dried pellet; Bacterial suspension; Agar plate
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.