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Lachnospiraceae bacterium — BNCC 354474

BNCC

Lachnospiraceae bacterium

SKU 354474

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismLachnospiraceae bacterium
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli).
Culture Method① Prepare 1-2 plates as specified above (pre-conditioned in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner. Immediately add sterile water to break the seal, then use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water (pre-conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Culture Conditions37 °C; 48-72 hours; anaerobic
Provided FormatsFreeze dried pellet; Agar plate
Culture Medium No.352241
Culture Medium NameColumbia blood agar plate
Culture Medium CompositionSpecial Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.