BNCC
J774A.1 – Mouse monocyte macrophages
SKU 338561
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | J774A.1 |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Macrophage-like，Round，Spindle-shaped (short)，Monolayer adherent growth |
| Culture Method | Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 °C refrigerator and place it in PE gloves. Quickly immerse it in a 37 °C water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 5-6mL of complete medium and place the flask in an incubator. Cell passage: ① Use a 5ml pipette to directly aspirate the culture medium and blow the cells; ② Observe under the microscope whether the cells are evenly dispersed. ③ Divide the cell suspension into fresh T25 flasks at a ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator.; ④ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches 80% -90%. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | cryovial; T25 culture flask |
| Culture Medium No. | 338068 |
| Culture Medium Name | DMEM-H Complete Medium (with 10% FBS) |
| Culture Medium Composition | 90%DMEM-H+10%FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.