BNCC
Human rhinovirus 30
SKU 381155
Bioware
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Human rhinovirus 30 |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Cryovial |
| Culture Method | ① Inoculation steps: 24-48 hours before inoculation, lay T25 bottles of host cells and prepare for virus inoculation when the host cell density reaches 80-90%; Remove the cell culture medium and inoculate 100-500 μL of virus solution, adsorb under culture conditions for 1-2 hours, gently shake the T25 bottle every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cell pathology; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution. The host cells are seeded in a 96 well plate and prepared for virus inoculation when the host cell density reaches 80-90%. ② Dilute the virus solution with complete culture medium in a 10 fold series, take 8 gradients of 10 ^ 1-10 ^ 8, and inoculate 100 μL per well quantitatively. Inoculate 6 wells at each dilution and culture according to the culture conditions. Regularly observe and record the pathological changes of cells at each dilution |
| Culture Conditions | 37 °C; 7 days; 5% CO2+95% air |
| Provided Formats | Cryovial |
| Culture Medium No. | 361988 |
| Culture Medium Name | EMEM Complete Medium (with 2% FBS) |
| Culture Medium Composition | 98% EMEM+2% FBS |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.