BNCC
Helicobacter felis Paster et al.
SKU 339474
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Helicobacter felis Paster et al. |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Morphological Characteristics | G− (red), spirochetes or cocci, Purity: Pure |
| Culture Method | ① Thaw Frozen vials in a 37°C water bath with vigorous shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to safety cabinet for handling; ③ Unscrew cap and aseptically transfer entire thawed culture to 5-6 mL brain heart infusate broth tube. ④ Inoculate 0.5 mL broth culture onto Agar slant; pour remaining culture onto Columbia blood agar plates and spread evenly. To achieve biphasic growth, loosely cap the slants and incubate at an angle. Gas supply is provided by a 2.5 L microaerophilic gas generator. Precautions: a. Use fresh, moist Columbia blood agar slants and plates; b. Biphasic growth on slants and plates should appear within 4-5 days, though turbidity is minimal. Growth can be observed via Gram staining of the broth; c. Upon obtaining robust growth, add equal volumes of 20% sterile glycerol to pooled broth from several biphasic slants, then freeze in Liquid nitrogen or at -80°C. |
| Culture Conditions | 37°C; 4-5 days; microaerophilic (3%-5% O₂, 10% CO₂) |
| Provided Formats | Frozen vial |
| Culture Medium No. | 352241 |
| Culture Medium Name | Columbia blood agar plate |
| Culture Medium Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -80°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.