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Fusobacterium nucleatum subsp. polymorphum (Knorr) Dzink et al.

SKU 311817

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismFusobacterium nucleatum subsp. polymorphum (Knorr) Dzink et al.
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsFusobacterium nucleatum polymorphic subspecies, Gram negative non spore forming anaerobic bacterium, slender in shape with spindle like tips at both ends, turbid in thioglycolate liquid culture medium, liquid culture is recommended
Culture Method① Prepare 1 tube of liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Culture Conditions37 °C; 4-5 days; anaerobic
Provided FormatsFreeze dried pellet; Bacterial suspension
Culture Medium No.353538
Culture Medium NameLiquid thioglycolate culture medium
Culture Medium CompositionTryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C2H3NaO2S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.