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Escherichiacoli(Migula)CastellanietChalmers — BNCC 335833

BNCC

Escherichiacoli(Migula)CastellanietChalmers

SKU 335833

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismEscherichiacoli(Migula)CastellanietChalmers
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; non-pigmented; Gram-negative (red); bacillus; purity: pure
Culture Method① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200 μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once colonies appear.
Culture Conditions37°C; 18-24h; Aerobic
Provided FormatsFreeze dried pellet; Slant culture; Bacterial suspension; Plate culture
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.