BNCC
Escherichia phageT5(Tequintavirus T5)
SKU 368071
Bioware
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Escherichia phageT5(Tequintavirus T5) |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Frozen vial |
| Culture Method | Host culture: 37 °C, aerobic, nutrient agar medium, 18-24h. Nutrient agar medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 20.0g agar (excluding liquid medium), 1.0L distilled water, pH 7.0. Sterilize at 121 °C for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50 mL nutrient broth medium, shake at 37 °C, 160-180 rpm until OD600 is 0.1-0.4; ② Transfer: Transfer an appropriate amount of host bacterial solution into the Agar plate, wait for the host bacterial solution to dry, and then apply bacteriophages onto the Agar plate; ③ Cultivation: Place the petri dish in a 37 °C incubator overnight for cultivation; ④ Bacterial culture: After 24 hours, plaques grow on the agar plate. Wash the agar plate and bacteriophages completely into a 40mL triangular flask and incubate overnight at 37 °C and 160-180rpm on a shaker. ⑤ Filtration: After 16-18 hours, centrifuge the bacteriophage culture at 4000g for 10 minutes and filter the lysate using a 0.22 μ m sterile filter. The filtrate can be stored at 4 °C |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | bacterial solution; Frozen vial tube |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.