BNCC
Escherichia phage T7
SKU 358500
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia phage T7 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Culture Method | Bacteriophage culture: ① Host bacterial preparation: Pick a single colony of host bacteria from a Agar plate, dissolve it in 40-50 mL nutrient broth medium, shake at 37 °C, 160-180 rpm until OD600 is 0.1-0.4; ② Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ④ Cultivation: Place the triangular flask at 37 °C and incubate overnight on a shaker at 160-180rpm; ⑤ Filtration: After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes, filter the lysate using a 0.2 μ m sterile filter, and store the filtrate at 4 °C |
| Culture Conditions | 37 °C; 18-24h; aerobic (recommended host bacterium BNCC356260) |
| Provided Formats | Freeze dried pellet; Bacterial liquid |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.