BNCC
Escherichia phage phiX174
SKU 358032
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia phage phiX174 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial tube |
| Culture Method | Nutrient broth medium: 3.0g beef extract, 10.0g peptone, 5.0g NaCl, 1.0L distilled water, pH 7.0. Sterilize at 121 °C for 15 minutes. Phage culture: ① Host bacterial preparation: Select a single colony of host bacteria from a Agar plate, dissolve it in 40-50mL nutrient broth medium, shake at 37 °C, 160-180 rpm until OD600 is around 0.4; ② Transfer: Remove the phage Frozen vial, rapidly melt it in a 37 °C water bath, and transfer all of it into the host bacterial solution mentioned above; ③ Cultivation: Place the triangular flask at 37 °C and shake at 160-180rpm overnight for cultivation; ④ Filtration: Centrifuge the phage culture at 4000g for 10 minutes, take the supernatant and collect the filtrate through a 0.22 μ m sterile filter. The filtrate can be stored at 4 °C |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Bacterial solution |
| Culture Medium No. | 369667 |
| Culture Medium Name | Nutrient Broth (NB) |
| Culture Medium Composition | Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.