BNCC
Escherichia coli Stbl4
SKU 353823
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli Stbl4 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colonies are pure, 1-2 mm, circular, convex, opaque, greyish-white colonies with entire margins and a smooth, glistening, moist texture that is easily picked up, composed of Gram-negative (red) bacilli. |
| Culture Method | 1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μL per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 352243 |
| Culture Medium Name | LB nutrient agar |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.