BNCC
Escherichia coli Stbl3(PLL3.7)
SKU 359014
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli Stbl3(PLL3.7) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Prepare 1 vial of sterile water and 2 x 90mm resistant plates; ② Disinfect ampoule surface, open in biosafety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve completely, then Agar plate onto antibiotic-resistant plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Streak plates and pick single colonies onto 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of bacterial culture as required by the experiment |
| Culture Conditions | 37°C; 18-24h; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 362905 |
| Culture Medium Name | LB Medium with 100μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.