BNCC
Escherichia coli Stbl3(pACT1: Cas9-GFP, U6: sgTK)
SKU 359700
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli Stbl3(pACT1: Cas9-GFP, U6: sgTK) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Culture Conditions | 37°C; 18-24h; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361029 |
| Culture Medium Name | LB Medium with 50μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | -80°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.