BNCC
Escherichia coli (Migula) Castellani and Chalmers
SKU 299217
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on both sides; centrally convex with a smooth, glossy surface; moist texture, easily liftable; G- (red), bacillus; purity: pure |
| Culture Method | ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears. |
| Culture Conditions | 37°C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.