BNCC
Escherichia coli (Migula) Castellani and Chalmers
SKU 391242
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Colony diameter: 2-3 mm; circular with neat margins; opaque, creamy white surface; centrally convex; smooth texture; viscous consistency; easily liftable; Gram-negative (red); bacillus; purity: pure |
| Culture Method | ① Prepare 1-2 plates as described above; ② Disinfect the ampoule surface, open it in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Immediately crush it with forceps; ③ Pipette 0.5 mL sterile water into the lyophilized tube. After fully dissolving the bacterial powder, dispense 200 μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Culture Conditions | 37°C; 18-24h; aerobic |
| Provided Formats | Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 363070 |
| Culture Medium Name | Tryptic Soy Agar (TSA) |
| Culture Medium Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.