BNCC
Escherichia coli M15(pREP4)
SKU 357914
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli M15(pREP4) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Round, irregular edges, opaque, gray white on the front and back, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Culture Method | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 385558 |
| Culture Medium Name | LB Agar Plate with 100μg/mL Kanamycin (Ready-to-use) |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.