BNCC
Escherichia coli K12 J53(RP4)
SKU 361521
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli K12 J53(RP4) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Purity: pure. |
| Culture Method | ① Prepare two plates as described above.② Disinfect the surface of the ampoule, open it in a biosafety cabinet, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, and transfer 200 μL of the suspension onto each of the two plates, spreading evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet |
| Culture Medium No. | 391054 |
| Culture Medium Name | LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, and 20μg/mL Tetracycline (Ready-to-use) |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.