BNCC
Escherichia coli HB101
SKU 357962
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli HB101 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture. No pigment is produced. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure. |
| Culture Method | ① Prepare 2 plates as described above.② Disinfect the surface of the ampoule, then open it in a biosafety cabinet. Briefly flame the top with an alcohol burner, immediately add sterile water to break the seal, and crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet |
| Culture Medium No. | 360873 |
| Culture Medium Name | LB Medium with 50μg/mL Streptomycin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.