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BNCC

Escherichia coli ETEC

SKU 186734

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismEscherichia coli ETEC
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsSingle colonies measure 1–2 mm in diameter, are circular in shape with neat, opaque edges, grayish-white in color, and exhibit a raised center.
Culture Method① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Culture Conditions37°C; 18-24h; aerobic
Provided FormatsAgar Slant; Bacterial suspension; Agar plate
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.