BNCC
Escherichia coli DH5α(pMK4)
SKU 392288
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli DH5α(pMK4) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial tube |
| Culture Method | ① Dissolve the Frozen vial tube in a water bath (37 °C) and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μL per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Frozen vial tube |
| Culture Medium No. | 361029 |
| Culture Medium Name | LB Medium with 50μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.