BNCC
Escherichia coli DH5α(pETBlue-2)
SKU 353856
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli DH5α(pETBlue-2) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | 1. Prepare one sterile liquid test tube (containing 5-10mL medium) and two 90mm plates; 2. After disinfecting the vial wall, open the aluminum cap and rubber stopper under sterile conditions. Pipette approximately 0.5 mL of liquid medium into the vial and gently tap to dissolve the bacterial pellet; 3. Once the pellet is fully dissolved, aspirate the entire solution back into the liquid test tube and mix thoroughly; 4. Pipette the mixture from the test tube onto plates, 200μL per well; 5. Incubate all test tubes and plates under the specified conditions. Do not seal or wrap plates. Observe for visible turbidity in the culture medium and colony or biofilm growth on plates after 18–24 hours; 6. If no growth is observed, continue incubation for up to 72 hours. Use the culture as required for the experiment. |
| Culture Conditions | 37°C; 18-24h; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361029 |
| Culture Medium Name | LB Medium with 50μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | -20°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.